| 1. | It enables a specific gene to be located on a particular restriction enzyme fragment .
它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。 |
| 2. | Large dna molecules are first dissected with restriction enzymes to produce specific fragments .
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。 |
| 3. | Restriction enzymes and reerse transcriptase are keys to gene engineering
限制性酶和逆转录酶成为基因工程的重要手段 |
| 4. | " for the discovery of restriction enzymes and their application to problems of molecular genetics
发现限制酶及其在分子遗传学方面的应用 |
| 5. | The recombi - nant plasmid pgex - hnadc3 was identified by dma sequencing and restriction enzyme mapping
限制性内切酶酶切及dna测序鉴定重组质粒。 |
| 6. | Large dna molecules are first dissected with restriction enzymes to produce specific fragments
首先用限制性内切酶将大的dna分子切断，产生出特殊的片段。 |
| 7. | It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正确，经酶切鉴定证实成功地构建了绿色荧光蛋白基因融合表达载体。 |
| 8. | Sequencing requires multiplecloned copies of the gene ; long dna sequences are cut into more manageable lengths using restriction enzymes
序列需要基因的多个克隆拷贝，用限制性酶可以把长的dna序列切成许多具所需长度的片段。 |
| 9. | To make pipetting easier , we will use an enzyme master mix that already contains the appropriate buffers and the two restriction enzymes
为了能在吸取液体时操作方便，我们会利用已经含有适当缓冲溶液与两种限制酶的酵素反应混合液来操作。 |
| 10. | The orf of the hbrp coding a peptide of 233 aa , using the dnasis v2 . 5 demo analysis its structure and pi 3 . screening 1 ) screening using restriction enzyme
Pcr产物和pgex一sx一1融合表达载体分别用bamhi和x五01限制性内切酶双酶切后连接，构建融合表达载体。 |
